Molecular Aspects of Myeloid Stem Cell
Development and Leukemia

Monday, May 23rd

12:00pm

LUNCH “Lemonade”

“Marble Foyer” (in front of P&G Hall) of the Aronoff Center for the Arts
650 Walnut St, Cincinnati, OH 45202
(directly across the street from the 21C Hotel)

12:50pm

LUNCH ENDS: please make your way to the auditorium

1:00pm

SESSION # 1 – CHAIR: Marie Dominique Filippi

Jarson-Kaplan Theater of the Aronoff Center for the Arts
650 Walnut St, Cincinnati, OH 45202
(directly across the street from the 21C Hotel)

1:00pm

1 Will

Iron-Dependent Lipid Turnover Confers Stem Cell Identity
Britta Will, Albert Einstein College of Medicine

1:20pm

2 Filippi

Divisional Memory Drives HSC Functional Diversity
Marie-Dominique Filippi, Cincinnati Children’s Hospital Medical Center

1:40pm

3 van Galen

Integrating Multiple Layers of Information from Single-Cell Sequencing in the Blood System
Peter van Galen, Harvard Medical School

2:00pm

4 Zon

Quality Assurance of Hematopoietic Stem Cells by Macrophages Determines Adult Stem Cell Clonality
Leonard Zon, Harvard University

2:20pm

5 Cancelas

Basolateral Complex Scribble is a Negative Regulator of Interferon-I Dependent Notch1 Activation in Adult Hematopoietic Stem Cells and Progenitors
Jose Cancelas, Cincinnati Children’s Hospital Medical Center

2:40pm

6 Mikkola

Decoding Mechanisms Governing Human Hematopoietic Stem Cell Specification and Self-Renewal
Hanna K.A. Mikkola, University of California Los Angeles

3:00pm

BREAK “Brownies and brownies and brownies”

3:30pm

SESSION # 2 – CHAIR: Claus Nerlov

Jarson-Kaplan Theater of the Aronoff Center for the Arts
650 Walnut St, Cincinnati, OH 45202
(directly across the street from the 21C Hotel)

3:30pm

7s Comazzetto

SHORT TALK
Vitamin C Limits Multipotent Progenitor Self-renewal and Clonal Expansion
Stefano Comazzetto, University of Texas Southwestern Medical Center

3:40pm

8 Krause

Post-Translational Modification of Runx1 Regulates Megakaryocyte-Erythroid Progenitor Fate Specification
Diane S. Krause, Yale University

4:00pm

9 Lucas

A Durable Anatomy with Local Microplasticity Enables Normal and Stress Hematopoiesis
Daniel Lucas, Cincinnati Children’s Hospital Medical Center

4:20pm

10s Silberstein

SHORT TALK
Myeloid-Biased HSC Require Semaphorin 4A from the Bone Marrow Niche for Self-Renewal Under Stress and Life-Long Persistence
Lev Silberstein, Fred Hutchinson Cancer Research Center

4:30pm

11s Reynaud

SHORT TALK
Hematopoietic Flt3+ Multipotent Progenitors Are Organized in Discrete
Functionally and Hierarchically Distinct Populations
Damien Reynaud, Cincinnati Children’s Hospital Medical Center

4:40pm

12 Nerlov

Fate-mapping of Hematopoietic Differentiation Pathways
Claus Nerlov, University of Oxford

5:00pm

13 Camargo

Resolving Embryonic Origins of the Blood through Cellular Barcoding
Fernando Camargo, Boston Children’s Hospital

5:20pm

14 Naik

A Revised ‘Parallel Tracks’ Landscape of Haematopoiesis Informed by Lentiviral and in Situ Cellular Barcoding
Shalin H. Naik, University of Melbourne

5:40pm

15 McKinney-Freeman

Discovering Novel Molecular Markers of Hematopoietic Stem Cell Functional Heterogeneity
Shannon McKinney-Freeman, St. Jude Children’s Research Hospital

6:00pm

TRAVEL OPTIONS

1. WALK 10 min/0.5 mile walk down Walnut Street to the riverfront
2. STREETCAR Cincinnati Bell connector is free
DESTINATION: National Underground Railroad Freedom Center
50 E Freedom Way, Cincinnati, OH 45202

6:30pm – 8:30pm

EVENING EVENT “39.0976° N, 84.5113° W The End of the South”

West Africa meets American South at the Mason-Dixon Line
(for reference see Netflix “High on the Hog”)

Tuesday, May 24th

6:00am – 7:50am

BREAKFAST (for guests of the 21C Hotel)

21C Hotel MAIN GALLERY (on the second floor of the hotel)

8:00am

SESSION # 3 – CHAIR: Steve Gore

Jarson-Kaplan Theater of the Aronoff Center for the Arts
650 Walnut St, Cincinnati, OH 45202
(directly across the street from the 21C Hotel)

8:00am

16 Gore

Progress Update on the National MDS Natural History Study
Steve Gore, NCI NIH

8:20am

17 Huang

Downregulation of Mitochondrial Complex II (MC II) in Myelodysplastic Syndromes
Gang Huang, University of Texas San Antonio

8:40am

18 Vyas

Quantitative Analysis of Clonal Selection and Stem Cell Expansion Combined with Single Cell Characterisation of Transcriptional and Chromatin Programs in Clonal Hemopoiesis
Paresh Vyas, University of Oxford

9:00am

19 DISCUSSION – ONE HOUR

Panel Discussion: Curing Myelodysplastic Syndrome (MDS): What are the most important questions?
Sponsor: The Taub Foundation
Discussion leader: Steve Gore

10:00am

BREAK

10:30am

SESSION # 4 – CHAIR: Jose Cancelas

SPONSOR: Hoxworth Blood Center
Jarson-Kaplan Theater of the Aronoff Center for the Arts
650 Walnut St, Cincinnati, OH 45202
(directly across the street from the 21C Hotel)

10:30am

20s Machlus

SHORT TALK
Regulation of Fatty Acids is a Novel Mechanism Controlling Megakaryopoiesis and Platelet Production
Kellie Machlus, Boston Children’s Hospital

10:40am

21 Tong

RAB27B Promotes Myeloid Malignancies through Regulating NRAS Palmitoylation, Trafficking and Signaling
Wei Tong, Children’s Hospital of Philadelphia

11:00am

22 Abkowitz

The transcriptomic landscape of normal and ineffective erythropoiesis at single cell resolution
Janis L Abkowitz, University of Washington

11:20am

23 Halene

ALKBH5 Controlled OGDH Stability Limits Hematopoietic Stem Progenitor Cell Metabolic Fitness – a m6A-Dependent Metabolic Rheostat
Stephanie Halene, Yale University School of Medicine

11:40am

24s Izraeli

SHORT TALK
The Conserved Proline 199 of the Transcription Factor ERG Mediates Its Leukemogenic Potential Through the Interactions with the NCoR-HDAC3 Co-Repressor Complex
Shai Izraeli, Schneider Children’s Medical Center of Israel

11:50am

25s Geiger

SHORT TALK
Clonal Hematopoiesis in the Elderly Might Not be Linked to Mutations in HSCs
Hartmut Geiger, University of Ulm

12:00pm

LUNCH “Smorbrod“

“Marble Foyer” (in front of P&G Hall) of the Aronoff Center for the Arts
650 Walnut St, Cincinnati, OH 45202
(directly across the street from the 21C Hotel)

1:00pm

SESSION # 5 – CHAIR: Harinder Singh

Jarson-Kaplan Theater of the Aronoff Center for the Arts
650 Walnut St, Cincinnati, OH 45202
(directly across the street from the 21C Hotel)

1:00pm

26 Rothenberg

Runx Transcription Factors and Epigenetic State Interactions as Drivers of Early T-cell Development
Ellen V. Rothenberg, California Institute of Technology

1:20pm

27 Singh

Discovery and Analysis of Composite Transcriptional Elements in Immune and Hematopoietic Cis-Regulomes
Harinder Singh, University of Pittsburgh

1:40pm

28 Rodriguez-Fraticelli

Identifying the Determinants of Stem Cell Aging Through Single-Cell Lineage Tracing
Alejo E. Rodriguez-Fraticelli, IRB Barcelona

2:00pm

29 Levine

Deciphering and Modeling Clonal Hematopoiesis and Evolution to MPN/AML
Ross L. Levine, Memorial Sloan Kettering Cancer Center

2:20pm

30 Steidl

Heparan Sulfate-Based Glycotyping for Cell Characterization and Isolation in the Hematopoietic System
Ulrich Steidl, Albert Einstein College of Medicine

2:40pm

31 Grimes

Isolating Discrete Hematopoietic Cell States
H. Leighton Grimes, Cincinnati Children’s Hospital Medical Center

3:00pm

BREAK “Sable et Fleurs”
**POSTER PRESENTERS GO GET YOUR POSTERS**

3:30pm

32s DISCUSSION

SHORT TALK
Neutrophils: Old cells with Neu Names?
Discussion leader: Lai Guan Ng

3:40pm

33 Speck

RUNX1 Attenuates Toll-like Receptor and Type I Interferon Signaling in Neutrophils
Nancy A. Speck, University of Pennsylvania

4:00pm

34 Hidalgo

Non-Canonical Neutrophils
Andres Hidalgo, Centro Nacional de Investigaciones Cardiovasculares (CNIC)

4:20pm

35 Ng

Neutrophil Heterogeneity in Tumors
Lai Guan Ng, A*STAR (Agency for Science, Technology and Research)

4:40pm

36 Ginhoux

Dendritic Cell Type 3 Arises from Ly6C+ Monocyte-Dendritic Cell Progenitors
Florent Ginhoux, Shanghai Jiao Tong University School of Medicine

5:00pm

**POSTER PRESENTERS GO FIRST ON THE SHUTTLES**
TRAVEL OPTIONS for other attendees

1. WAIT for the shuttles to return
1. WALK 16 min/0.7 mile
2. STREETCAR Cincinnati Bell connector is free
DESTINATION: Cincinnati Music Hall
1241 Elm St, Cincinnati, OH 45202

5:30pm – 7:00pm

POSTER SESSION

SPONSOR: EVANS MDS (a funding initiative of the Edward P Evans Foundation)
on the 2^nd floor of the EDYTH B. LINDNER GRAND FOYER of the Cincinnati Music Hall

7:00pm – 9:00pm

EVENING EVENT “Quinceanera“

The traditional celebration of a Mexican girl’s 15th birthday.
on the 1st floor of the EDYTH B. LINDNER GRAND FOYER of the Cincinnati Music Hall

Wednesday, May 25th

6:00am – 7:50am

BREAKFAST (for guests of the 21C Hotel)

21C Hotel MAIN GALLERY (on the second floor of the hotel)

8:20am

SESSION # 6 – CHAIR: Emmanuelle Passegué & Michael Milsom

Jarson-Kaplan Theater of the Aronoff Center for the Arts
650 Walnut St, Cincinnati, OH 45202
(directly across the street from the 21C Hotel)

8:20am

37 Tenen

Myeloid Transcription Factors are Really Rna Binding Proteins Facilitating Enhancer Promoter Interactions
Daniel G. Tenen, Harvard

8:40am

38 Waskow

Human Innate Immunity in NSGW41 Mice
Claudia Waskow, Leibniz Institute on Aging – Fritz Lipmann Institute

39 Kapur —cancellation—-

Calcium Channel Blockers Inhibit Obesity Induced Exacerbated Clonal Hematopoiesis
Reuben Kapur, Indiana University School of Medicine

9:00am

40 Milsom

Next Generation Interrogation of DNA Methylome Data Focussed Upon Analysis of Co-Ordinately-Programmed CpGs (CP-CpGs) During Hematopoietic Differentiation
Michael Milsom, HI-STEM: The Heidelberg Institute for Stem Cell Technology and Experimental Medicine

9:20am

41 Trowbridge

Distinct TNF Receptors Dictate Hematopoietic Stem Cell Fitness Versus Lineage Output in Dnmt3a-Mutant Clonal Hematopoiesis During Aging
Jennifer J. Trowbridge, The Jackson Laboratory

9:40am

42 Goodell

Requirement and Function of the Disordered N-terminal Domain of DNMT3A in Development
Margaret A. Goodell, Baylor College of Medicine

10:00am

BREAK

10:30am

43s Viny _recorded from quarantine_

SHORT TALK
Epigenetic Determinates of HSC Self-Renewal and Lineage Fate Commitment
Aaron D. Viny, Columbia University Irving Medical Center

10:40am

44 King

The Contribution of Hematopoietic Stem and Progenitor Cells to Trained Immunity
Katherine Y. King, Baylor College of Medicine

11:00am

45 Starczynowski

The Role of Microbial-Derived Signals in Myeloid Malignancies
Daniel T. Starczynowski, Cincinnati Children’s Hospital Medical Center

11:20am

46 Aifantis

Inflammation Remodels the Immune Microenvironment in Acute Myeloid Leukemia
Iannis Aifantis, NYU Grossman School of Medicine

11:40am

47 Beaudin

Heterogeneity of Fetal Hematopoietic Stem and Progenitors Shapes the Prenatal Response to Inflammation
Anna E. Beaudin, University of Utah School of Medicine

12:00pm

LUNCH “Jerusalem“

“Marble Foyer” (in front of P&G Hall) of the Aronoff Center for the Arts
650 Walnut St, Cincinnati, OH 45202
(directly across the street from the 21C Hotel)

1:00pm

SESSION # 6 CONTINUES

Jarson-Kaplan Theater of the Aronoff Center for the Arts
650 Walnut St, Cincinnati, OH 45202
(directly across the street from the 21C Hotel)

1:00pm

48 Gazit

Acute and Chronic Immune Stimuli of Hematopoietic Stem Cells
Roi Gazit, Ben-Gurion University of the Negev

1:20pm

49 Passegue

Autophagy Counters Inflammation-Driven Glycolytic Impairment in Aging Hematopoietic Stem Cells
Emmanuelle Passegué, Columbia University

1:40pm

SESSION # 7 – CHAIR: Len Zon

Jarson-Kaplan Theater of the Aronoff Center for the Arts
650 Walnut St, Cincinnati, OH 45202
(directly across the street from the 21C Hotel)

1:40pm

50 Dick

A Cellular Hierarchy Framework for Understanding Heterogeneity and Predicting Drug Response in Acute Myeloid Leukemia
John E. Dick, Princess Margaret Cancer Centre

2:00pm

51s Eisfeld

SHORT TALK
Genomic Landscape for Black Patients with Acute Myeloid Leukemia (AML)
Ann-Kathrin Eisfeld, The Ohio State University

2:10pm

52s Guzman

SHORT TALK
Genomic Landscape for Hispanic Patients with Acute Myeloid Leukemia (AML)
Monica L. Guzman, Weill Cornell Medicine

2:20pm

53 Papapetrou

A Pan-AML Panel of Human iPSCs
Eirini P Papapetrou, Icahn School of Medicine at Mount Sinai

2:40pm

54 Stegmaier

Discovery of New AML Therapeutic Targets with Functional Genomics Approaches
Kimberly Stegmaier, Dana-Farber Cancer Institute

3:00pm

LONG BREAK “Koriela“

3:50pm

55s Skorski

SHORT TALK
DNA Polymerase Theta Protects Leukemia Cells from Metabolic-Induced DNA Damage
Tomasz Skorski, Temple University Lewis Katz School of Medicine

4:00pm

56 Nimer

Tyrosine Phosphorylation of Carm1 Promotes Its Enzymatic Activity and Alters Its Target Specificity
Stephen D. Nimer, University of Miami

57 Delwel —cancellation—-

EVI1 is a Repressor of CEBPA Transcription via a Distal Enhancer in inv(3)/t(3;3) AML
Ruud Delwel, Erasmus MC Cancer Institute and Oncode Institute

58 Figueroa —cancellation—-

sPDZD2, a Novel Soluble Tumor Suppressor in AML
Maria E. Figueroa, University of Miami Health System

4:20pm

59 Hiebert

Defining the RUNX1/ETO Transcriptional Control Circuits
Scott W. Hiebert, Vanderbilt University School of Medicine

4:40pm

60 Bresnick

Pathogenic Mutation that Dislocates GATA2 Zinc Fingers Establishes a Hematopoiesis-Disrupting Signaling Network
Emery H. Bresnick, Wisconsin Blood Cancer Research Institute

5:00pm

61 Zheng

RUNX1 Mutation Causes Megakaryocyte-Primed Hematopoietic Stem Cell Differentiation Blockage and Familial Platelet Disorder
Yi Zheng, Cincinnati Children’s Hospital Medical Center

5:20pm

62 Bryder

Mechanisms of Disease Progression in MLL-ENL Driven AML
David Bryder, Lund University

5:40pm

63 Bonifer

Gene Regulatory Network Dynamics in Acute Myeloid Leukemia
Constanze Bonifer, University of Birmingham

6:00pm

64 Trumpp

Decoding Transcriptomic and Epigenetic Consequences of Chromosomal Rearrangements in Ck-Aml at Single-Cell Resolution
Andreas Trumpp, Heidelberg Institute for Stem Cell Technology and Experimental Medicine

6:20pm

65s Velten

SHORT TALK
Hunting leukemic stem cells with clonally resolved single-cell multi-omics
Lars Velten, Centre for Genomic Regulation, Barcelona Spain

6:30pm

TRAVEL OPTIONS

WALK 5 min/0.3 mile
DESTINATION: The Mercantile Library
414 Walnut St #1100, Cincinnati, OH 45202

7:00pm – 9:00pm

EVENING EVENT “Isle of Capri“

A quiet evening of guitar, good wine and pasta in a setting from 1835.

Thursday, May 26th

6:00am – 7:50am

BREAKFAST (for guests of the 21C Hotel)

21C Hotel MAIN GALLERY (on the second floor of the hotel)

8:00am

SESSION 8 CHAIR: Ari Melnick

Jarson-Kaplan Theater of the Aronoff Center for the Arts
650 Walnut St, Cincinnati, OH 45202
(directly across the street from the 21C Hotel)

8:00am

66 Ernst

Regulation of B/Myeloid Fate to Improve Murine Models of High Risk, MLL-rearranged Pediatric B-ALL
Patricia Ernst, Children’s Hospital Colorado

8:20am

67 Bernt

The Role of GATA2 in Drug Resistance in AML
Kathrin Bernt, Children’s Hospital of Philadelphia

8:40am

68 Doulatov

Nuclear Lamins in Clonal Progression to High-Risk Myeloid Neoplasms
Sergei Doulatov, University of Washington

9:00am

69 Bhatia

Adaptive Metabolic Response to Tyrosine Kinase Inhibition in Chronic Myelogenous Leukemia Stem Cells
Ravi Bhatia, University of Alabama at Birmingham (UAB)

9:20am

70s Cockerill

SHORT TALK
Defining the Gene Regulatory Networks Targeted by RUNX1 and FLT3 Inhibitors
Peter Cockerill, University of Birmingham

9:30am

71s Wiest

SHORT TALK
The ERK2 DBP Domain Opposes Pathogenesis of a JAK2V617F-Driven Myeloproliferative Neoplasm
David L. Wiest, Fox Chase Cancer Center

9:40am

BREAK

10:30am

72s Jones

SHORT TALK
SIRT3 Inhibition Targets Leukemia Stem Cells Through Perturbing Fatty Acid Metabolism
Courtney L. Jones, University of Toronto

10:40am

73 Carroll

Acute Myeloid Leukemia with Mutant TP53 Requires Mevalonate Biosynthesis for Chemotherapy Resistance
Martin Carroll, Children’s Hospital of Philadelphia

11:00am

74 Kentsis

Epigenetic Mechanisms and Therapeutic Targeting of Leukemia Stem Cell Quiescence
Alex Kentsis, Sloan Kettering Institute

11:20am

75 Xu

Dissecting Mechanisms of Acquired Resistance to IDH Inhibition in Myeloid Leukemia
Jian Xu, UT Southwestern Medical Center

11:40am

76 Melnick

Identifying Genetic Susceptibilities to Epigenetic Therapies for Rational Targeted Therapy of AML Patients
Ari Melnick, Weill Cornell Medicine

12:00pm

LUNCH “Ploughman’s Lunch”
BOX LUNCH TAKEAWAY

1:00pm

BUS 1 will transport directly to CVG

1:30pm

BUS 2 will transport directly to CVG